Tom Wojtunik, who penned the evening, will direct with music direction by Justin Fischer.
York alumni assembled for the concert include Klea Blackhurst, Liam Forde, , , , , Christine Baird, Erin Eichfeld, Kevin Lagasse, Justin Luciano, Zakk Mannella and MacKenzie Perpich.
The program will be a sampler of songs from Main Stage productions presented over the past 45 years, including The Golden Apple, Enter Laughing, Closer Than Ever, Yank!, Storyville and nine musicals.
A second anniversary concert is scheduled for Jan. 19, 2015.
54 Below is located at 254 West 54th Street. There is a $40 cover charge and $25 food and beverage minimum. Tickets and information are available at www.54Below.com.